Tip Page: MUSC DNA Microarray DB home>manage>Stat_Snap_Shot
You may view and download ()publicly available microarray data in the MUSC database by selecting the "List of all Publicly Available cRNA Records" link below. To download data, select the icon.
Contents of MUSC DNA Microarray Database
The following tables summarize the content of the MUSC DNA microarray
database (records tabulated by specific categories are linked):
Summary of the Public Availability of cRNA Records in the Database:
871 of 1595 cRNA record sets are currently available.
11 of 1595 cRNA record sets will be available within 6 months.
6 of 1595 cRNA record sets will be available within 1 year.
707 of 1595 cRNA record sets are not yet scheduled for public release.
0 cRNA record sets have become available after a 6 month hold period.
597 cRNA record sets have become available after a 1 year hold period.
Note that each cRNA record in the database is a set of files corresponding to experimentation derived from the hybridization of an individual cRNA preparation to a single microarray. For example, an Affymetrix MAS 5.0 record set includes files having .DAT, .CHP, .EXP, .CEL and .RPT extensions. Each record set also includes a tab-delimited file (i.e., .TXT) containing expression values resulting from transformation of the raw data. GCOS files (.EXP and .DAT files) are contained within a compressed data file, called a cabinet file (.CAB).
List of all publicly available data in the database. cRNA target data present in the database selected on the following
mining criteria: Organism: [All] ,
Tissue: [All] ,
Cell Line [All] .
To view and download associated files
click the symbol
in the right column.
Analysis of the effects of fibulin-1 splice variants on gene expression - II.
Project Title: Analysis of the effects of fibulin-1 splice variants on gene expression - II.
Record No: _1034702317.490542
Contributor(s): W. Scott Argraves
Project Description: Alternative splicing of fibulin-1 RNA results in the production of two major splice variants (designated fibulin-1C and D). DNA microarray analysis will be use to determine the profile of genes influenced by the expression of the individual fibulin-1 variants. RNA will be analyzed from HT-1080 human fibrosarcoma cells (ATCC# CCL-121) stably transfected with the empty vector pcDNAneo-I or with the same vector designed to express either human fibulin-1C or fibulin-1D variants.
Project Title: Chronic Alcohol Treatment of Mice
Record No: _1036182891.833255
Contributor(s): Peter W. Kalivas, Jacqueline F. McGinty, Howard Becker
Project Description: To compare the pattern of gene expression in four different groups of mice: ED; chronic ethanol exposure and withdrawal. Followed by a chance of weekly ethanol drinking.
END; chronic ethanol exposure and withdrawal, but no chance to access to ethanol any more.
CD; same treatment as ED, but no chronic ethanol vapor exposure. They still have any chance to drink ethanol at corresponding times.
CND; same treatment as END, but no chronic ethanol vapor exposure. Namely, these animals are ethanol naive.
Brain tissue is collected 7 days after chronic ethanol exposure in END group and about 18 hr after last drink in ED and CD groups. Hippocampus (Hp) is the main brain region for studying the mechanism of alcohol dependence.
Project Title: Cocaine self administration
Record No: _1040158907.359895
Contributor(s): Jacqueline F Mcginty
Project Description: To study alterations in gene expression in 3 brain regions (medial prefrontal cortex, nucleus accumbens, and caudate-purtamen (CPu) of rats trained to self administer cocaine (0.25 mg/kg/infusion) or saline (0.05 ml/infusion), 22 hours and 3 weeks after the last drug session.
Project Title: Amphetamine Administration
Record No: _1041526307.490529
Contributor(s): Jacqueline F. Mcginty
Project Description: Male, Sprague-Dawley rats (12 weeks of age, 250-275g, Charles River Labs) received a single intraperitoneal injection of saline or 5 mg/kg amphetamine and were decapitated 3 hr later. Equithesin (1ml/100gm) was administered 5 minutes before decapitation. The striatum was dissected manually from a 2 mm coronal section.
117
Rat [Rat Genome U34A ] Tissue, Normal/Non-Cancer
Brain: Striatum
061202 A Ctl Sal1
Noise (RawQ):
1.580
_1041626610.935383
118
"
" Sal3
1.540
_1041627470.728561
119
"
Exp Amp2
1.650
_1041626684.299734
120
"
" Amp4
1.540
_1041627201.215098
121
"
090302 B Ctl Sal5
1.610
_1041627263.721669
122
"
" Sal7
2.200
_1041627372.469229
123
"
Exp Amp6
1.630
_1041627302.528768
124
"
" Amp8
1.680
_1041627419.253028
Steroid-induced cataract formation
Project Title: Steroid-induced cataract formation
Record No: _1042145125.901060
Contributor(s): Eric James
Project Description: To determine the gene expression profile in lens epithelial cells (LECs) treated with steroid. RNA was isolated from B3 human LECs treated with dexamethasone and untreated control B3 LECs.
Influence of Oxidized and Glycated LDL on Pericyte Gene Expression
Project Title: Influence of Oxidized and Glycated LDL on Pericyte Gene Expression
Record No: _1042840345.675738
Contributor(s): Timothy J. Lyons, W. Scott Argraves, Jamie L. Barth
Project Description: To determine the effects of normal low density lipoprotein (LDL), highly oxidized and glycated LDL (HOG) and glycated LDL (GLDL) on the gene expression of cultured human pericyte.
Evaluate the effect of RAP on sonic hedgehog (Shh)-responsive genes in endoderm-like BN cells.
Project Title: Evaluate the effect of RAP on sonic hedgehog (Shh)-responsive genes in endoderm-like BN cells.
Record No: _1043193512.965289
Contributor(s): W. Scott Argraves
Project Description: To determine sonic and indian hedgehog responsive genes in Brown Norway Rat Endoderm-like (BN) cells. The study involves isolation of RNA from control BN cells, BN cells treated with Shh, BN cells treated with the LDL receptor family antagonist RAP and BN cells treated with Shh and RAP. A major goal of thes studies is to determine if megalin, a member of the LDL receptor family, influences the expression of hedgehog responsive genes.
Transcriptional Regulation of Angiogenesis by Ets Family of Transcritpiton Factors
Project Title: Transcriptional Regulation of Angiogenesis by Ets Family of Transcritpiton Factors
Record No: _1043428127.139575
Contributor(s): Robin C. Muise-Helmericks
Project Description: Determine the functional redundance of target gene selection by the E6 Transforming Sequence (ETS) family of transcription factors.
Pathways mediating sodium-calcium exchanger gene upregulation in response to exchanger inhibition.
Project Title: Pathways mediating sodium-calcium exchanger gene upregulation in response to exchanger inhibition.
Record No: _1043428779.797282
Contributor(s): Donald R. Menick
Project Description: To determine the gene expression profiles of RNAs isolated from hearts of adult mice (2-3 months of age) having the sodium-calcium exchanger promoter driving luciferase. This gene expression profile will be compared to that of the same transgenic mouse strain injected with KB-R7943 (sodium-calcium exchanger inhibitor) twice a day for 3 days.
Effect of suberonylanilide hydroxamic acid (SAHA) on mesangial cell gene expression
Project Title: Effect of suberonylanilide hydroxamic acid (SAHA) on mesangial cell gene expression
Record No: _1043430017.828750
Contributor(s): Gary S. Gilkeson
Project Description: This project seeks to test the effects of the histone deacetylase inhibitor suberonylanilide hydroxamic acid (SAHA) on mesangial cell gene expression.
Analysis of the effects of fibulin-1 splice variants on gene expression - I.
Project Title: Analysis of the effects of fibulin-1 splice variants on gene expression - I.
Record No: _1044051067.741580
Contributor(s): W. Scott Argraves, Liam Gallagher, Waleed O. Twal
Project Description: The goal of this project is to determine the effects of expressing the two major splice variants (fibulin1C and fibulin-1D) on gene expression in HT1080 fibrosarcoma and MDA MB 231 breast carcinoma cells.
190
Human [Human HuGeneFL HU6800] Cell Line, Transformed/Tumor, Transfected
Fibrosarcoma: HT1080
Project Title: Spinal cord injury-induced pain behaviors
Record No: _1044636266.563500
Contributor(s): Jacqueline F Mcginty
Project Description: The goal of this aim is to determine whether the expression level of genes in the anterior cingulate cortex, retrosplenial cortex, periaqueductal gray, and medial thalamus are altered in mice that exhibit spontaneous pain behaviors. Thirty-six mice will be anesthetized, undergo laminectomy, and receive an injection of 0.6 ul PBS (n=12) or 0.6 ul 75 mM QUIS (n=24) as described below. An excess number of mice will be injected so that enough RNA will be made available when the QUIS-injected mice are divided into 2 groups based on grooming behavior. On each post-surgical day, the mice will be evaluated for emergence of pain-related behavior, i.e., excessive grooming. On average, 60% of mice develop excessive grooming behavior between days 8-12 when spinal cord lesions that spare lamina I reach a critical size. On day 14, the mice will be anesthetized and decapitated, and the spinal cords will be harvested for histological lesion analysis. The brains will be frozen until RNA is extracted from the selected brain areas as described below. Total RNA pooled from the same brain regions of 3 mice will be reverse transcribed, labeled, and hybridized to a Clontech mouse plastic array. Three arrays will be hybridized per brain area of each group. After the arrays are scanned by phosphorimaging, they will be aligned with AtlasImage software and the hybridization signal intensities will be normalized and compared between PBS-injected, QUIS-injected mice that exhibited excessive grooming, and QUIS-injected mice that did not exhibit grooming. Mice will undergo surgery and intraspinal injections of QUIS or PBS as described above. Post-surgical behavior will be evaluated over the same time course and the mice will be anesthetized and decapitated on day 14.
Gene expression profiling of murine allantois development/vascularization
Project Title: Gene expression profiling of murine allantois development/vascularization
Record No: _1046388476.368830
Contributor(s): W. Scott Argraves, Christopher Drake
Project Description: This project seeks to determine the profile of gene expression during development/vascularization of the mouse allantois. At 7.5 days post coitum (dpc) the murine allantois is devoid of blood vessels and at 9.5 dpc it is highly vascularized. Therefore, profiling will be done on samples prepared from 7.5, 8.5 and 9.5 dpc mouse allantoides.
Gene Expression in the Retinoid X Receptor Alpha-Deficient Mouse
Project Title: Gene Expression in the Retinoid X Receptor Alpha-Deficient Mouse
Record No: _1048540658.231106
Contributor(s): Steve Kubalak
Project Description: To determine genes that are dysregulated in the hearts of mouse embryos deficient in the expression of the retinoid X receptor alpha (RXR alpha-null mice).
238
Mouse [Murine Genome U74Av2 ] Tissue, Normal/Non-Cancer
Heart: Ventricle, AV and Outflow Tract (No Atria)
031103 A Exp RXRaHet
Noise (RawQ):
2.410
_1048541373.310272
239
"
032703 A Ctl RXRaWT
3.140
_1048717527.475187
240
"
Exp RXRaKO
2.110
_1048717616.422288
241
"
033103 B Ctl RXRaWT
2.070
_1049744058.321254
242
"
Exp RXRaKO
2.190
_1049744178.502258
Effects of ethanol on gene expression in the developing rat brain
Project Title: Effects of ethanol on gene expression in the developing rat brain
Record No: _1051198881.339370
Contributor(s): Lawrence Judson Chandler, Jeremy Barth
Project Description: To determine the effects of ethanol on gene expression in the developing rat brain, five day old rat pups were injected intraperitoneally with saline or ethanol (3g/kg x2). The hippocampus and cerebellum were then collected after 6 hrs.
243
Rat [Rat Neurobiology U34 ] Tissue, Normal/Non-Cancer
Brain: Cerebellum
080901 A Ctl CB-2
Noise (RawQ):
0.990
_1051199886.667027
244
Brain: Hippocampus
" HP-1
1.510
_1051199532.327879
245
Brain: Cerebellum
Exp CB-4
1.130
_1051199555.439950
246
Brain: Hippocampus
" HP-3
1.390
_1051200020.917113
247
Brain: Cerebellum
100101 B Ctl CB-10
NoRptFile
_1051200405.943340
248
"
" CB-9
NoRptFile
_1051200365.389210
249
Brain: Hippocampus
" HP-5
NoRptFile
_1051200494.880648
250
"
" HP-6
NoRptFile
_1051200239.184464
251
Brain: Cerebellum
Exp CB-11
NoRptFile
_1051200459.903379
252
"
" CB-12
NoRptFile
_1051200063.785772
253
Brain: Hippocampus
" HP-7
NoRptFile
_1051200309.512757
254
"
" HP-8
NoRptFile
_1051200117.667104
255
Brain: Cerebellum
032602 C Ctl CB-1
Noise (RawQ):
1.200
_1051200534.907800
256
"
" CB-6
1.100
_1051200848.510536
257
Brain: Hippocampus
" HP-10
2.280
_1051200583.124204
258
"
" HP-2
1.360
_1051200728.308336
259
"
" HP-9
1.710
_1051200901.316671
260
Brain: Cerebellum
Exp CB-3
1.090
_1051200759.965887
261
"
" CB-7
0.880
_1051200875.194898
262
Brain: Hippocampus
" HP-11
0.940
_1051200645.394754
263
"
" HP-12
0.410
_1051200679.967616
264
"
" HP-4
1.300
_1051200813.388954
265
Brain: Cerebellum
042003 D Ctl CB-18
2.420
_1052936977.605868
266
"
" CB-20
2.430
_1052937168.712574
267
Brain: Hippocampus
" HP-18
2.630
_1052936246.653047
268
"
" HP-20
1.720
_1052936738.749367
269
Brain: Cerebellum
Exp CB-19
3.890
_1052937057.412191
270
"
" CB-21a
2.120
_1052937226.151464
271
"
" CB-21b
3.420
_1052937332.780783
272
Brain: Hippocampus
" HP-19
1.900
_1052936713.641714
Role of Fli-1 in transformation and differentiation
Project Title: Role of Fli-1 in transformation and differentiation
Record No: _1052158620.132887
Contributor(s): Dennis K. Watson
Project Description: Molecular analyses of transcriptional changes that occur in mice harboring defined mutations.
Project Title: GDNF Knockout Study
Record No: _1059490943.520078
Contributor(s): Jacqueline F Mcginty, Lotta Granholm
Project Description: The purpose of this study was to identify changes in gene expression in the striatum and prefrontal cortex of Glial cell line-Derived Neurotrophic Factor (GDNF) heterozygous (+/-) mice on a C57BL/6 background. The striatum was dissected from 4 wild-type (GDNF +/+) and 4 heterozygous (GDNF +/-) mice at 12 months of age. Total RNA was extracted, reverse transcribed, labeled and hybridized to GeneChips.
Hyaluronan Induced Gene Expression in MCF7/Adr cells
Project Title: Hyaluronan Induced Gene Expression in MCF7/Adr cells
Record No: _1062175684.220401
Contributor(s): Shibnath Ghatak, Suniti Misra, Bryan Toole
Project Description: This study seeks to determine the changes in gene expression of drug-resistant human mammary carcinoma cells (MCF7/Adr) resulting from treatment with hyaluronan oligomers. MCF7/Adr cells were grown in culture for 24 hours followed by a further 24 hours in the presence or absence of 100 ug/ml hyaluronan oligomers. The cells were harvested and the RNA was isolated for use in microarray analysis.
286
Human [Human Genome U133A ] Cell Line, Transformed/Tumor
Breast Carcinoma: MCF-7/Adr
081303 A Ctl C2A
Noise (RawQ):
4.180
_1064009072.492688
287
"
" C4A
3.400
_1064009140.818519
288
"
Exp T1A
2.890
_1064004149.416825
289
"
" T2A
3.510
_1064004174.905837
290
"
" T4A
3.460
_1064009002.613461
Effect of in vitro glycated LDL on gene expression of human aortic endothelial cells
Project Title: Effect of in vitro glycated LDL on gene expression of human aortic endothelial cells
Record No: _1068758003.383716
Contributor(s): Richard L Klein
Project Description: Low density lipoproteins (LDL) from diabetic patients may be divided into glycated and non-glycated subfractions using affinity chromatography. Previous studies indicate that glycated LDL alters the metabolism of human monocyte-derived macrophages. We have determined in preliminary studies that the glycated LDL subfraction alters the secretion of tissue plasminogen activiator (tPA) and plasminogen activator inhibitor-1 (PAI-1) from human aortic endothelial cells. We will determine that these LDL subfractions alter the expression of these two genes. We will determine the effect of the glycated LDL subfraction compared to the non-glycated LDL subfraction isolated from the same diabetic patient on the expression of an array of human aortic endothelial genes. Total LDL from the diabetic patient from whom the LDL subfractions were obtained as well as pooled LDL from non-diabetic donors are used as controls. A lipoprotein free incubation (Basal) is also included as a control.
291
Human [Human Genome U133A ] Cell Line, Primary
Aorta: Endothelial: HAEC
010504 A Ctl 11D
Noise (RawQ):
5.410
_1068767896.488445
292
"
" 1G
3.260
_1068763979.186990
293
"
" 6R
3.310
_1068766803.446948
294
"
Exp 10R
3.120
_1068767777.604600
295
"
" 12D
3.560
_1068767986.474031
296
"
" 13D
3.780
_1068768107.659442
297
"
" 14D
4.050
_1068768288.206469
298
"
" 15D
4.690
_1068768379.919612
299
"
" 2G
3.550
_1068763997.198910
300
"
" 3G
3.640
_1068766271.944905
301
"
" 4G
3.380
_1068766523.489908
302
"
" 5G
2.950
_1068766666.892038
303
"
" 7R
4.010
_1068766949.600467
304
"
" 8R
3.640
_1068767529.434840
305
"
" 9R
3.460
_1068767693.588931
Analysis of the effects of fibulin-1 variants on gene expression - III
Project Title: Analysis of the effects of fibulin-1 variants on gene expression - III
Record No: _1073508551.760725
Contributor(s): W. Scott Argraves
Project Description: The goal of this project is to determine the effects that each of the two major fibulin-1 splice variants (i.e., fibulin-1C and fibulin-1D) have on gene expression in HT1080 fibrosarcoma cells and MDA MB 231 breast carcinoma cells.
306
Human [Human Genome U133A ] Cell Line, Transformed/Tumor, Transfected
Breast carcinoma: MDA-MB-231
010604 A Ctl Control
Noise (RawQ):
2.280
_1073511246.469954
307
"
Exp fibulinC
2.280
_1073508921.908931
308
"
" fibulinD
2.430
_1073510805.725384
309
"
010604 B Ctl Control
2.410
_1074122224.573030
310
"
Exp fibulinC
2.190
_1074121347.668737
311
"
" fibulinD
3.040
_1074122028.679870
312
"
031104 C Ctl Control1
2.220
_1079631576.835992
313
"
" Control2
1.810
_1079631708.297029
314
"
040104 D Ctl Control
2.320
_1080945329.682590
315
"
Exp fibulinC
2.150
_1080945463.925911
316
"
" fibulinD
2.090
_1080945641.105246
Characterization of the effects of zero gravity on mouse gut gene expression
Project Title: Characterization of the effects of zero gravity on mouse gut gene expression
Record No: _1077142794.504304
Contributor(s): Jeremy L Barth, Ted Bateman (Clemson University), W. Scott Argraves (MUSC)
Project Description: The aim of this project is to characterize the changes in small intestine gene expression that result from a zero gravity environment. Combined ileum and jejunum tissue samples from mice flown in the space shuttle (STS-108) for 12 days will be compared to the same tissues collected from control animals maintained on earth. Intestinal tissues collected from animals were flushed and then snap forzen in liquid nitrogen.
Analysis of the effects of fibulin-1 splice variants on gene expression - IV.
Project Title: Analysis of the effects of fibulin-1 splice variants on gene expression - IV.
Record No: _1082480601.462457
Contributor(s): W. Scott Argraves
Project Description: The goal of this project is to determine the effects that each of the two major fibulin-1 splice variants (i.e., fibulin-1C and fibulin-1D) have on gene expression in HT1080 fibrosarcoma cells and MDA MB 231 breast carcinoma cells.
Influence of in vitro glycated of LDL on endothelial cell gene expression
Project Title: Influence of in vitro glycated of LDL on endothelial cell gene expression
Record No: _1086191314.486415
Contributor(s): Richard L Klein
Project Description: To determine whether in vitro glycated normal low density lipoprotein (N-LDL) from a pool of donors will alter human aortic endothelial cell gene expression. In particular we are interested in effects on tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1). RNA from cells in basal conditions (no treatment with in vitro glycated LDL) will be used as controls. RNA from the in vitro glycated LDL cells will be compared with RNA from cells treated with N-LDL. The length of treatment of the human aortic endothelial cells (HAEC) with glycated LDL or N-LDL was 6 hours.
330
Human [Human Genome U133A ] Cell Line, Primary
Aorta: Endothelial: HAEC
040204 A Ctl 10.Basal
Noise (RawQ):
3.280
_1086191834.195920
331
"
" 11.Basal
4.270
_1086192102.350070
332
"
" 12.Basal
3.330
_1086192955.109913
333
"
Exp 13.IvGly
2.940
_1086193113.168889
334
"
" 14.IvGly
3.120
_1086193259.598650
335
"
" 15.IvGly
3.000
_1086193522.764277
336
"
" 16.N-LDL
2.900
_1086193795.659618
337
"
" 17.N-LDL
3.200
_1086193974.122968
338
"
" 18.N-LDL
3.340
_1086194143.139522
Alcohol Drinking Study 2004-2005 (Prefrontal Cortex)
Project Title: Alcohol Drinking Study 2004-2005 (Prefrontal Cortex)
Record No: _1090438859.735354
Contributor(s): Howard Becker, Jacqueline McGinty
Project Description: Gene/Protein Analysis of Ethanol Relapse: The series of studies will utilize
an optimum mouse model of ethanol consumption and withdrawal to identify
molecular neuroadaptive changes underlying increased relapse susceptibility.
Microarrays will be used to detect unique changes in gene and protein
expression related to ethanol relapse susceptibility.
Expression profiling of the periostin null mouse - I
Project Title: Expression profiling of the periostin null mouse - I
Record No: _1092353761.960453
Contributor(s): Simon J. Conway
Project Description: To compare mRNA expression profiles in organs of periostin-deficient and wildtype adult mice.
Gene Profiling of Atrioventricular Junction of the Embryonic Mouse
Project Title: Gene Profiling of Atrioventricular Junction of the Embryonic Mouse
Record No: _1093383095.811006
Contributor(s): Andy Wessels, Scott Argraves
Project Description: This project seeks to determine the profile of gene expression during atrioventricular (AV) cardiac cushion development and during AV conduction development.
Project Title: Muscularization of Cardiac Mesenchymal Cells
Record No: _1093885114.684144
Contributor(s): Andy Wessels
Project Description: This project seeks to compare the gene expression profile of mesenchymal cells in the cardiac outflow tract (OFT) before muscularization, at stage E12.0, and after muscularization, at stage E15.0.
Analysis of the effects of fibulin-1C deficiency on MEF gene expression
Project Title: Analysis of the effects of fibulin-1C deficiency on MEF gene expression
Record No: _1094676616.142469
Contributor(s): W. Scott Argraves, Waleed Twal, Marion Cooley
Project Description: The purpose of this study was to determine the effects of abrogating fibulin-1C variant expression on the profile of gene expression in mouse embryo fibroblasts (MEFs). MEFs were generated from wild-type and fbln1 exon 15-/- embryos (E13.5). RNA was isolated from cultures of each type of cell and Affymetrix DNA microarray hybridization performed.
Gene expression in transgenic mouse model of prostate cancer
Project Title: Gene expression in transgenic mouse model of prostate cancer
Record No: _1094890182.119155
Contributor(s): James S. Norris
Project Description: To develop doxycycline-inducible transgenic mouse model that represents the broad spectrum of human prostate cancer. RTTA (reverse tetracycline-dependent transactivat) and EcoRI transgenic mice were generated by Gina Keller in the Norris lab. Genomic instability was induced by transgene expression. The mouse model is used to study mechanisms involved in the early stages of prostate carcinogenesis.
Effects of S-(1,2-dichlorovinyl)-L-cysteine on cultured human kidney cells
Project Title: Effects of S-(1,2-dichlorovinyl)-L-cysteine on cultured human kidney cells
Record No: _1096905527.251063
Contributor(s): Rick Schnellmann
Project Description: The aim of this project is to search for gene changes in the human renal cortex following exposure to S-(1,2-dichlorovinyl)-L-cysteine (DCVC). Renal cortical cells were isolated by collagenase digestion, cutlured for 10 days until confluent and then exposed to a low non-toxic dose of 0.1uM or a dose close to a toxic level of 1uM. The aim is to seek genes that may be altered following subchronic exposure that may be useful as markers of change prior to injury.
381
Human [Human Genome U133A ] Tissue, Normal/Non-Cancer
Kidney: cortex
092304 A Ctl HK18con
Noise (RawQ):
3.010
_1096905934.166270
382
"
Exp HK180.1D
2.280
_1096906505.733633
383
"
" HK181.0D
2.170
_1096906532.499467
384
"
020705 B Ctl HK20Con
2.090
_1109882116.616703
385
"
" HK22Con
2.010
_1109882919.130534
386
"
" HK23Con
2.020
_1109882987.630359
387
"
Exp HK200.1D
2.290
_1109882452.859868
388
"
" HK201.0D
2.080
_1109883169.331959
389
"
" HK220.1D
2.400
_1109882681.643687
390
"
" HK221.0D
2.180
_1109883224.734968
391
"
" HK230.1D
1.980
_1109882777.780272
392
"
" HK231.0D
2.180
_1109883278.422007
Role of Fibulin-1 C in cardiac tricuspid valve development
Project Title: Role of Fibulin-1 C in cardiac tricuspid valve development
Record No: _1098115324.131494
Contributor(s): W. Scott Argraves, Waleed Twal, Marion Cooley
Project Description: The goal of this project is to determine the effects that the loss of fibulin-1 C splice variant have on gene expression of cells isolated from fibulin-1 exon 15 -/- targeted knockout mice tricuspid valve explants.
Characterization of the effects of zero gravity on mouse muscle gene expression.
Project Title: Characterization of the effects of zero gravity on mouse muscle gene expression.
Record No: _1112121233.298086
Contributor(s): Jeremy L Barth, Ted Bateman, W. Scott Argraves
Project Description: The aim of this project is to characterize the changes in skeletal muscle (gastrocnemius) gene expression that result from a zero gravity environment. Gastrocnemius tissue samples from mice flown in the space shuttle (STS-108) for 12 days will be compared to the same tissues collected from control animals maintained on earth in animal enclosure modules (AEM). Muscle tissues collected from animals were snap frozen in liquid nitrogen.
Project Title: Demyelination using hexachlorophene
Record No: _1112637729.714026
Contributor(s): Laree Hiser
Project Description: Mouse embryonic stem cells (ES-D3) are differentiated using retinoic acid to form mixed cultures of CNS-like neurons and glial cells. Cultures are grown on Matrigel for approximately 15 days to allow myelin formation. A short exposure to hexachlorophene (HCP) causes demyelination. RNA is isolated from cultures at various times after treatment with HCP. Cultures differentiated at the same time but exposed to NaOH rather than HCP are used as myelinated controls.
Project Title: Identification of periostin-responsive genes
Record No: _1112979109.299832
Contributor(s): Simon J. Conway
Project Description: The purpose of this study was to identify changes in gene expression in two cell lines (P19Cl6 & NIH3T3) that both endogenously express periostin. The P19Cl6 cell line are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes. Whilst NIH3T3 cells are a mouse fibroblast cell line. P19Cl6 cells were induced to differentiate with DMSO then transfected using lipoamine after a further 2 days growth. P19Cl6 cells were transfected with either empty pcDNA3.1 vector (sample #A1), pax3-HA tagged full length cDNA (sample #B2) or mutant pax3-HA tagged full length cDNA (sample #C3) – and the cells were harvested after an additional 2 days after transfection - then the RNA was isolated for use in microarray analysis. NIH3T3 cells were transfected via lipoamine with either empty pcDNA3.1 vector (sample #D4) or full length periostin cDNA (sample #E5) – and the cells were harvested after a further 2 days growth and the RNA was isolated for use in microarray analysis.
Expression profiling of the periostin null mouse - II
Project Title: Expression profiling of the periostin null mouse - II
Record No: _1112979422.354171
Contributor(s): Simon J. Conway
Project Description: The purpose of this study was to identify changes in gene expression in the developing heart (including both atria, ventricles and outflow tracts) of 10.5, 12.5 dpc & newborn periostin null (-/-) vs. wild type littermates on a 129SvxC57BL/6 background. Embryonic 10.5 dpc hearts were dissected from 15 wild-type (+/+, # F6) and 11 null (-/-, #G7) littermate embryos resulting from heterozygous intercrosses.
Embryonic 12.5 dpc hearts were dissected from 10 wild-type (+/+, #H8) and 6 null (-/-, #I9) littermate embryos resulting from heterozygous intercrosses.
Newborn hearts were dissected from 3 wild-type (+/+, #J10) and 3 null (-/-, #K11) 2 day old neonate littermates resulting from heterozygous intercrosses.
Total RNA was extracted, reverse transcribed, and labeled.
Ethanol withdrawal induced gene expression changes in hippocampus
Project Title: Ethanol withdrawal induced gene expression changes in hippocampus
Record No: _1119483506.364734
Contributor(s): Jacqueline F McGinty, Howard Becker, Roberto Melendez
Project Description: The aim of this project is to determine the profile of gene changes in hippocampus of C57 mice exposed to multiple ethanol withdrawals.
Project Title: Cocaine self administration 24 hr
Record No: _1119653081.765121
Contributor(s): Jacqueline F McGinty
Project Description: To study alterations in gene expression in 6 brain regions (dorsal prefrontal cortex, ventral prefrontal cortex, nucleus accumbens shell, nucleus accumbens core, ventral tegment area and substantia nigra) of rats trained to self administer cocaine (0.25 mg/kg/infusion) or saline (0.05 ml/infusion), 24 hours after the last drug session.
Ethanol withdrawal induced gene expression changes in prefrontal cortex.
Project Title: Ethanol withdrawal induced gene expression changes in prefrontal cortex.
Record No: _1121752779.420651
Contributor(s): Jacqueline F McGinty, Howard Becker, Roberto I. Melendez
Project Description: The aim of this project is to determine the profile of gene changes in prefrontal cortex of C57 mice exposed to multiple ethanol withdrawals.
Ethanol withdrawal induced gene expression changes in ventral striatum
Project Title: Ethanol withdrawal induced gene expression changes in ventral striatum
Record No: _1127845242.426722
Contributor(s): Jacqueline F McGinty, Howard Becker, Roberto I Melendez
Project Description: The aim of this project is to determine the profile of gene changes in ventral striatum of C57 mice exposed to multiple ethanol withdrawals.
Project Title: Gene expression profile in bone marrow
Record No: _1129750495.548983
Contributor(s): Kaidi Mikhitarian
Project Description: Determine gene expression profile in 2 normal bone marrow samples and 2 bone marrow samples from breast cancer patients
480
Human [Human Genome U133A ] Tissue, Normal/Non-Cancer
Bone marrow
101705 A Ctl N-BM1
NoRptFile
_1129751160.192199
481
"
" N-BM2
NoRptFile
_1129751404.410570
482
Human [Human Genome U133A ] Tissue, Cancer
Bone marrow
Exp BM-G785
NoRptFile
_1129751071.511046
483
"
" BM-G961
NoRptFile
_1129750794.968921
oxLDL-IC (immune complexes) and Foam Cell formation
Project Title: oxLDL-IC (immune complexes) and Foam Cell formation
Record No: _1143236307.138644
Contributor(s): Samar M. Hammad
Project Description: The experiment designed to test the hypothesis oxLDL-IC differentially regulate the expression of genes involved in foam cell formation, activation, and cytokine release.
484
Human [Human Genome U133 Plus 2.0 ] Cell Line, Transformed/Tumor
Histiocytic Lymphoma: U-937
031706 A Ctl Agg-IgG
_1143497967.904883
485
"
" KLH-IC
_1143498124.443272
486
"
" PBS
_1143317889.669640
487
"
Exp ox-LDL
_1143319576.216219
488
"
" oxLDL-IC
_1143319756.153410
489
"
" RbIgG-IC
_1143498636.476357
490
"
" RbSer-IC
_1143498718.643441
491
"
031706 B Ctl Agg-IgG
_1143497450.712900
492
"
" KLH-IC
_1143498278.914942
493
"
" PBS
_1143318861.582416
494
"
Exp ox-LDL
_1143319340.508903
495
"
" oxLDL-IC
_1143320454.918140
496
"
" RbIgG-IC
_1143498401.946839
497
"
" RbSer-IC
_1143499000.732656
Vincristine and Noscapine
Project Title: Vincristine and Noscapine
Record No: _1144864750.509209
Contributor(s): Laree Hiser
Project Description: Mouse embryonic stem cells (ES-D3) are differentiated using retinoic acid to form mixed cultures of CNS-like neurons and glial cells. Cultures are grown on Matrigel for approximately 15 days to allow myelin formation.Demyelination is observed in cell culture after treatment with vincristine while noscapine appears to provide protection against demyelination. Microarray analysis will be used to help determine the mechanisms behind these neurotoxic and neuroprotective effects.
Project Title: Cocaine Self Administration
Record No: _1156961280.368353
Contributor(s): Jacqueline F McGinty
Project Description: To study alterations in gene expression in two brain regions: nucleus accumbens, and caudate putamen of rats trained to self administer cocaine (0.25 mg/kg/infusion) or saline (0.05 ml/infusion) three weeks after the last drug session.
502
Rat [Rat Expression 230A ] Tissue, Normal/Non-Cancer
Brain: Caudate Putamen
082806 A Ctl CPu1
NoRptFile
_1156962929.269777
503
"
" CPu2
NoRptFile
_1156962988.511458
504
"
" CPu3
NoRptFile
_1156963057.265941
505
"
" CPu4
NoRptFile
_1156963088.708081
506
Brain: Nucleus Accumbens
" NAc1
NoRptFile
_1156962332.830499
507
"
" NAc2
NoRptFile
_1156962538.375026
508
"
" NAc3
NoRptFile
_1156962653.365355
509
"
" NAc4
NoRptFile
_1156962686.884936
510
Brain: Caudate Putamen
Exp CPu6
NoRptFile
_1156963124.871780
511
"
" CPu7
NoRptFile
_1156963165.754756
512
"
" CPu8
NoRptFile
_1156963200.586560
513
"
" CPu9
NoRptFile
_1156963231.644887
514
Brain: Nucleus Accumbens
" NAc6
NoRptFile
_1156962736.368820
515
"
" NAc7
NoRptFile
_1156962805.196633
516
"
" NAc8
NoRptFile
_1156962847.876790
517
"
" NAc9
NoRptFile
_1156962880.722000
Cocaine Self Administration
Project Title: Cocaine Self Administration
Record No: _1156961940.628164
Contributor(s): Jacqueline F McGinty
Project Description: To study alterations in gene expression in three brain regions: medial prefrontal cortex, nucleus accumbens, and caudate putamen of rats trained to self administer cocaine (0.25 mg/kg/infusion) or saline (0.05 ml/infusion) twenty two hours after the last drug session.
518
Rat [Rat Expression 230A ] Tissue, Normal/Non-Cancer
Brain: Caudate Putamen
081706 A Ctl CPu1
Noise (RawQ):
3.290
_1156963558.936510
519
"
" CPu2
3.150
_1156963777.930377
520
"
" CPu3
3.590
_1156963819.285491
521
"
" CPu4
2.990
_1156963855.784062
522
Brain: Nucleus Accumbens
" NAc1
3.220
_1156964648.632025
523
"
" NAc2
3.680
_1156964734.597793
524
"
" NAc3
3.900
_1156964763.250180
525
"
" NAc4
3.450
_1156964797.751630
526
Brain: Prefrontal Cortex
" PFC1
2.550
_1156964195.841847
527
"
" PFC2
3.220
_1156964285.614306
528
"
" PFC3
3.100
_1156964312.922871
529
"
" PFC4
3.410
_1156964352.393499
530
Brain: Caudate Putamen
Exp CPu5
2.920
_1156963900.938966
531
"
" CPu6
3.630
_1156964050.563627
532
"
" CPu7
2.820
_1156964113.372577
533
"
" CPu8
2.670
_1156964139.315639
534
Brain: Nucleus Accumbens
" NAc5
4.180
_1156964826.288230
535
"
" NAc6
8.670
_1156964861.742993
536
"
" NAc7
3.770
_1156964891.433320
537
"
" NAc8
3.970
_1156964921.275615
538
Brain: Prefrontal Cortex
" PFC5
3.230
_1156964468.944270
539
"
" PFC6
3.700
_1156964528.500746
540
"
" PFC7
3.380
_1156964566.105595
541
"
" PFC8
3.650
_1156964600.338722
mGluR5 antagonist treatment
Project Title: mGluR5 antagonist treatment
Record No: _1157475916.505123
Contributor(s): M. Foster Olive
Project Description: This project is aimed at identifying novel signaling proteins that interact with the type 5 metabotropic glutamate receptor (mGluR5), which has been implicated in psychiatric disorders such as anxiety, depression and drug addiction. Rats were treated twice daily with either vehicle (20% cyclodextrin) or the mGluR5 antagonists 2-methyl-6-(phenylethynyl)-pyridine (MPEP) or 3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]-pyridine (MTEP), and 1 hr following the last drug administration, rats were euthanized and the frontal cortex was dissected and prepared for microarray analysis.
542
Rat [Rat Expression 230A ] Tissue, Normal/Non-Cancer
Brain: Frontal Cortex
092106 A Ctl Vehicle
Noise (RawQ):
2.740
_1159983517.541480
543
"
Exp MPEP
2.520
_1159983563.314913
544
"
" MTEP
2.640
_1159983586.821630
545
"
112206 B Ctl Vehicle2
3.120
_1165610038.902502
546
"
" Vehicle3
3.350
_1165610068.950260
547
"
" Vehicle4
3.410
_1165610112.255162
548
"
" Vehicle5
2.810
_1165610162.317840
549
"
Exp MPEP2
3.340
_1165609711.536741
550
"
" MPEP3
3.630
_1165609901.253077
551
"
" MPEP4
3.110
_1165609952.324254
552
"
" MPEP5
3.070
_1165609986.398323
553
"
" MTEP2
3.220
_1165609505.985697
554
"
" MTEP3
5.210
_1165609558.746085
555
"
" MTEP4
3.090
_1165609598.862799
556
"
" MTEP5
2.420
_1165609633.715803
48hr Ethanol Withdrawal Hippocampus
Project Title: 48hr Ethanol Withdrawal Hippocampus
Record No: _1159808789.487426
Contributor(s): Jacqueline F McGinty, Howard C. Becker, Roberto I. Melendez
Project Description: Gene expression changes in the hippocampus of C57BL/6J mice following 48 hr of chronic ethanol vapor exposure.
Enhanced Chemotherapy Effects by Upregulation of Oxidative Stress Response Genes by NOV-002, a Nove
Project Title: Enhanced Chemotherapy Effects by Upregulation of Oxidative Stress Response Genes by NOV-002, a Nove
Record No: _1160068654.469906
Contributor(s): Alberto Montero
Project Description: The central hypothesis of the proposed research is that: NOV-002, a novel stabilized formulation of oxidized glutathione, is a chemotherapy adjuvant that results in synergistic anti-tumor activity in vivo, when combined with taxanes and anthracyclines in breast cancer animal models. Consequently, we hypothesize that NOV-002 enhances the effect of chemotherapy by upregulating oxidative stree response genes.
567
Human [Human Genome U133 Plus 2.0 ] Cell Line, Transformed/Tumor
Breast: Ductal Carcinoma
092606 A Ctl 453cont
Noise (RawQ):
2.260
_1160068752.745762
568
"
Exp 453N002
0.900
_1160068861.975769
Chick HLHS
Project Title: Chick HLHS
Record No: _1161593148.783120
Contributor(s): David Sedmera, Michal Mrug, Angela C. deAlmeida
Project Description: Analysis of gene expression in the embryonic chick heart during development and after hemodynamic perturbations (COBRE).
Affymetrix Gene Chip analysis of methamphetamine injected GDNF heterozygous and wild type mice
Project Title: Affymetrix Gene Chip analysis of methamphetamine injected GDNF heterozygous and wild type mice
Record No: _1164750673.561451
Contributor(s): Jacqueline F McGinty
Project Description: At 2.5 months of age, five GDNF heterozygous and five wild type mice were each injected with 10 mg/kg methamphetamine (i.p.), and five GDNF heterozygous and five wild type mice were each injected with 0.2 ml saline (i.p.) (n=10 per group; 20 total). The injections were given four times at two hour intervals. Two weeks post injection (at 3 months of age), the mice were sacrificed and the brains were extracted. The brains were dissected and the striata of each was frozen for RNA extraction and further analysis using the Affymetrix Gene Chips.
Project Title: Prostate cancer: AC-overexpression cell lines
Record No: _1169586790.198523
Contributor(s): Xiang Liu
Project Description: DU145 cells transfected with Acid ceramidase-GFP and stable clones were obtained by G418 screen.
613
Human [Human Genome U133 Plus 2.0 ] Cell Line, Transformed/Tumor, Transfected
Prostate Tumor: DU145
011007 A Ctl 7
Noise (RawQ):
1.560
_1170135076.233263
614
"
" 8
1.810
_1170135191.238740
615
"
" 9
1.850
_1170135266.357553
616
"
071107 B Ctl Lxc
2.890
_1182448211.344027
617
"
040809 C Ctl 2
2.120
_1238529111.829729
618
Prostate Tumor: PC3
" 3
2.100
_1238529218.107290
619
Prostate Tumor: Dupro
" 5
2.190
_1238529610.105602
620
Prostate Tumor: PPC1
" 7
2.390
_1238529769.576731
621
"
" 9
2.360
_1238530072.145060
Head and Neck Tissue with AC Upregulation
Project Title: Head and Neck Tissue with AC Upregulation
Record No: _1169587419.726212
Contributor(s): Xiang Liu
Project Description: WE are testing gene net work in head and neck tumor tissues with up-regulated expression of acid ceramidase, compared with tumor tissues with normal expression of Acid ceramidase. WE also analyze adjacent normal tissues as base line control. Sample starts with "T" represents tumor tissue and "N" represents normal tissue.
622
Human [Human Genome U133 Plus 2.0 ] Tissue, Normal/Non-Cancer
Head and Neck Tissue
032807 A Ctl 16
Noise (RawQ):
1.600
_1182442651.657428
623
"
" 18
1.630
_1182444415.836075
624
"
" 28
1.990
_1182445467.799200
625
"
" 29
2.080
_1182445622.600335
626
"
" 30
1.960
_1182445714.209074
627
"
" 33
2.120
_1182445796.656045
628
"
" 35
2.340
_1182445927.772925
Diabetes and Periodontal Gene Expression
Project Title: Diabetes and Periodontal Gene Expression
Record No: _1178742267.333968
Contributor(s): Yan Huang, Dr. John Sanders, Dr. Elizabeth Slate
Project Description: It has been well established that diabetic patients have increased severity of periodontal disease as compared to nondiabetic patients. However, the underlying mechanism remains unknown. The specific objective of this project is to determine the periodontal expression of inflammatory cytokines and other genes involved in periodontal disease in diabetic patients.
629
Human [Human Genome U133 Plus 2.0 ] Tissue, Pathological/Non-Cancer
Periodontal tissue
022807 A Exp B
Noise (RawQ):
1.320
_1178744632.883704
630
"
" D
1.240
_1178744883.777372
631
"
" E
1.270
_1178744945.667012
632
"
" F
1.340
_1178745003.413455
633
"
" G
1.360
_1178745056.268315
634
"
042707 A Exp A
1.090
_1178743380.160866
635
"
011508 B Exp 1
2.200
_1201557428.336147
636
"
" 2
1.580
_1201558017.873438
637
"
" 3
1.250
_1201558187.512652
638
"
" 4
1.150
_1201558258.763490
639
"
" 5
1.430
_1201558084.756360
640
"
" 6
1.010
_1201558295.837331
NOVELOS: The Effects of NOV-002 and PABA/NO on HL60
Project Title: NOVELOS: The Effects of NOV-002 and PABA/NO on HL60
Record No: _1180470532.661811
Contributor(s): Ken Tew, Danyelle Townsend
Project Description: HL60 cells were separated into four (4) treatment groups: Control (no drug), 300 uM NOV-002 for 5 days, PABA/NO 1X, and PABA/NO Chronic. The RNA was extracted using the TRIzol/RNeasy Hybrid Protocol (5/25/2007).
NOV-002 is a myeloproliferative agent that has been shown to increase the mean survival in NSCL and Ovarian cancer patients. The mechanism is unknown, but previous studies show that bone marrow and nephrotoxicity are reduced in patients receiving this drug treatment in combination with Cisplatin and Paclitaxel. PABA/NO is a known nitrosative stress-inducer which leads to apoptosis, and so we are looking at the effects NOV-002 has on cells when treated with this drug.
641
Human [Human Genome U133 Plus 2.0 ] Cell Line, Transfected
Leukemia: HL 60
061107 A Ctl Control
Noise (RawQ):
2.060
_1180470916.141082
642
"
Exp NOV002
2.090
_1180471364.387429
643
"
" PABANO
2.050
_1180471497.447014
644
"
" PABANOch
2.030
_1180471599.692905
Comparative Analysis of Crtl1 deficient and wild type hearts at ED10.5
Project Title: Comparative Analysis of Crtl1 deficient and wild type hearts at ED10.5
Record No: _1188927354.269820
Contributor(s): Andy Wessels
Project Description: RNA was isolated from Crtl1 deficient and wild type littermate hearts at embryonic day 10.5. The entire heart was isolated and snap frozen in liquid nitrogen and stored at -70C. RNA was isolated using the Qiagen RNeasy minikit. Six different Crtl1+/- x Crtl1+/- litters were collected and 2 hearts (each from different litters) were combined to create 3 wild type samples and 3 knockout samples. These samples were then amplified and hybridized to the Affymetrix mouse genome chips.
Project Title: Study of Galectin-3 in Reproduction
Record No: _1189115179.814278
Contributor(s): SAMIR RAYCHOUDHURY
Project Description: To utilize microarray analyses to evaluate the differences in testicular gene expression between male galectin-3 null mice and wildtype mice.
Alcohol Drinking Study 2007-2008 (Prefrontal Cortex)
Project Title: Alcohol Drinking Study 2007-2008 (Prefrontal Cortex)
Record No: _1192817286.681080
Contributor(s): Howard Becker, Roberto I Melendez
Project Description: Gene/Protein Analysis of Ethanol Relapse: The series of studies will utilize an optimum mouse model of ethanol consumption and withdrawal to identify molecular neuroadaptive changes underlying increased relapse susceptibility. Microarrays will be used to detect unique changes in gene and protein expression related to ethanol relapse susceptibility.
Project Title: Alcohol Drinking Study 2007-2008 (Hippocampus)
Record No: _1192821225.935019
Contributor(s): Howard Becker, Roberto I Melendez
Project Description: Gene/Protein Analysis of Ethanol Relapse: The series of studies will utilize an optimum mouse model of ethanol consumption and withdrawal to identify molecular neuroadaptive changes underlying increased relapse susceptibility. Microarrays will be used to detect unique changes in gene and protein expression related to ethanol relapse susceptibility.
Role of Sphingolipids in Yeast Oxidative Stress Response
Project Title: Role of Sphingolipids in Yeast Oxidative Stress Response
Record No: _1194272896.801463
Contributor(s): L. Ashley Cowart
Project Description: This project involves the observation of gene expression of control and oxidatively stressed samples of 5 strains of yeast: Jk93d[alpha] (WT), Lcb4, Lcb5, Lcb4/5, Dpl1 mutants.
Project Title: ABT-737/PV10
Record No: _1197397981.243109
Contributor(s): Jin H Song
Project Description: It is hypothesized that anticancer immune responses might be enhanced by exposure of target cells to agents that inhibit apoptosis. These experiments were performed to determine how transcriptional responses are altered in a renal carcinoma cell (KV10) in response to treatment with ABT-737, which is an inhibitor of the antiapoptotic oncoprotein Bcl-2.
742
Human [Human Genome U133 Plus 2.0 ] Cell Line, Primary
Kidney: carcinoma: KV10
121207 A Ctl CTRL1
_1197655593.756835
743
"
" CTRL2
_1197657347.206560
744
"
" CTRL3
_1197657388.796797
745
"
Exp ABT1
Noise (RawQ):
1.750
_1197585088.535092
746
"
" ABT2
_1197655499.302189
747
"
" ABT3
_1197655535.518648
Strain on Chick Limb Bud Mesenchyme
Project Title: Strain on Chick Limb Bud Mesenchyme
Record No: _1205171050.331644
Contributor(s): Jay D Potts
Project Description: Chick limb bud mesnchyme mRNA isolated from Stages 22 and 25 with and without mechanical stretch during culture
Project Title: Gene expression signature induced by EBV-dUTPase
Record No: _1207934675.249788
Contributor(s): Maria-Eugenia Ariza
Project Description: Epstein-Barr virus (EBV) is an oncogenic gamma herpesvirus that infects a significant percentage (>90%) of the population worldwide. Following primary infection, EBV establishes latency in memory B-cells where it can be reactivated during the lifetime of the individual. EBV is the causative agent of infectious mononucleosis and is implicated in the pathogenesis of a variety of lymphoproliferative disorders and human malignancies, particularly in immune-suppressed transplant recipients and in patients with congenital or acquired immunodeficiency. While the latent forms of EBV are known to be responsible for carcinogenesis, little is known regarding the possible role that lytic replication of the virus may contribute to this process. Our preliminary data demonstrated that dUTPase, a protein encoded by EBV during lytic replication, activates NF-kB in adose-dependent manner through TLR2 and myeloid differentiation factor 88 (MyD88). Furthermore, EBV-dUTPase stimulated a strong pro-inflammatory cytokine response in human macrophages and peripheral blood mononuclear cells (PBMCs). However, the mechanism(s) by which EBV-dUTPase activates cellular signaling cascades leading to NF-kB activation and induction of pro-inflammatory cytokines is not known. The overall hypothesis is that the immunomodulatory functions of EBV-dUTPase contribute to the aberrant immune responses and pathophysiology of diseases caused by EBV infection by interacting with and activating the TLR2/MyD88 signaling pathway(s) on human dendritic cells.
760
Human [Human Genome U133 Plus 2.0 ] Cell Line, Primary
Dendritic cells
4/3/08 A Ctl hDCCtl1
Noise (RawQ):
2.290
_1207934841.180124
761
"
" hDCCtrl2
_1207934949.359968
762
"
Exp dUPTase1
_1207935033.829753
763
"
" dUTPase2
_1207935126.858824
764
"
" dUTPase3
_1207935201.217112
Prostate cancer tissues
Project Title: Prostate cancer tissues
Record No: _1215696076.130575
Contributor(s): Xiang Liu
Project Description: Comparison of patient-matched normal/tumor prostate tissues. Samples obtained from the tumor bank were analyzed by microarray for evaluation of differential gene expression. The aim of this experiment is to identify genetic elements among high acid ceramidase (AC) expressing tumors against low AC expressing tumors and normal tissues.
765
Human [Human Genome U133 Plus 2.0 ] Tissue, Normal/Non-Cancer
Prostate: Epithelium
061208 A Ctl 1
Noise (RawQ):
1.950
_1215696388.223595
766
Human [Human Genome U133 Plus 2.0 ] Tissue, Cancer
Prostate: Epithelium
" 10
1.980
_1215701469.865549
767
"
" 12
2.230
_1215701494.529316
768
"
" 14
2.120
_1215701542.169874
769
Human [Human Genome U133 Plus 2.0 ] Tissue, Normal/Non-Cancer
Prostate: Epithelium
" 15
1.580
_1215701562.128408
770
"
" 17
1.900
_1215701582.297000
771
Human [Human Genome U133 Plus 2.0 ] Tissue, Cancer
Prostate: Epithelium
" 18
1.140
_1215701604.465361
772
Human [Human Genome U133 Plus 2.0 ] Tissue, Normal/Non-Cancer
Prostate: Epithelium
" 5
1.540
_1215701229.460642
773
"
" 7
1.860
_1215701320.603359
774
Human [Human Genome U133 Plus 2.0 ] Tissue, Cancer
Prostate: Epithelium
" 8
1.990
_1215701368.516993
775
Human [Human Genome U133 Plus 2.0 ] Tissue, Normal/Non-Cancer
Prostate: Epithelium
" 9
1.990
_1215701439.883981
Analysis of Gene Expression Profiles in Prefrontal Cortex of Major Depressive Disorder
Project Title: Analysis of Gene Expression Profiles in Prefrontal Cortex of Major Depressive Disorder
Record No: _1219077343.946690
Contributor(s): Mark C Austin
Project Description: This study is intended to test the overall hypothesis that subjects with major depression will exhibit gender-specific gene expression profiles in the prefrontal cortex relative to their gender-matched control subjects.
776
Human [Human Genome U133 Plus 2.0 ] Tissue, Normal/Non-Cancer
Brain: Prefontal Cortex
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